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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
04/06/1998 |
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Data da última atualização: |
20/07/2010 |
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Autoria: |
LOWE, A. J.; HANOTTE, O.; GUARINO, L. |
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Título: |
Standardization of molecular genetic techniques for the characterization of germplasm collections: the case of random amplified polymorphic DNA (RAPD). |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Plant Genetic Resources Newsletter, n. 107, p. 50-54, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly responsible for variation in the generation of RAPD fragments, and examine ways to reduce these sources of unwanted variation. We propose a table containing a collation of all the descriptors thought necessary to describe the conditions and assessment procedures necessary for reproducible RAPD profiling of plant material. The standardization of procedure and presentation of results should facilitate the comparison of results both between and within laboratories working on similar material, and allow RAPDs to be used as a standard germplasm descriptor. |
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Palavras-Chave: |
Characterization of germplasm collection; PCR method; Technique standardibation. |
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Thesagro: |
Genética Molecular; Germoplasma. |
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Thesaurus Nal: |
reproducibility. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01563naa a2200217 a 4500
001 1052533
005 2010-07-20
008 1996 bl --- 0-- u #d
100 1 $aLOWE, A. J.
245 $aStandardization of molecular genetic techniques for the characterization of germplasm collections$bthe case of random amplified polymorphic DNA (RAPD).
260 $c1996
520 $aRandom amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly responsible for variation in the generation of RAPD fragments, and examine ways to reduce these sources of unwanted variation. We propose a table containing a collation of all the descriptors thought necessary to describe the conditions and assessment procedures necessary for reproducible RAPD profiling of plant material. The standardization of procedure and presentation of results should facilitate the comparison of results both between and within laboratories working on similar material, and allow RAPDs to be used as a standard germplasm descriptor.
650 $areproducibility
650 $aGenética Molecular
650 $aGermoplasma
653 $aCharacterization of germplasm collection
653 $aPCR method
653 $aTechnique standardibation
700 1 $aHANOTTE, O.
700 1 $aGUARINO, L.
773 $tPlant Genetic Resources Newsletter$gn. 107, p. 50-54, 1996.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
